TY - JOUR T1 - Effects of Diets with Different Proportions of Protein/Carbohydrate on Retinal Manifestations in <em>db</em> Mice JF - In Vivo JO - In Vivo SP - 265 LP - 272 VL - 32 IS - 2 AU - EMI ARIMURA AU - HIDEAKI OKATANI AU - TOMOAKI ARAKI AU - MIHARU USHIKAI AU - MIWA NAKAKUMA AU - MASAHARU ABE AU - HIROAKI KAWAGUCHI AU - HIROYUKI IZUMI AU - MASAHISA HORIUCHI Y1 - 2018/03/01 UR - http://iv.iiarjournals.org/content/32/2/265.abstract N2 - Background/Aim: Diabetic nephropathy is aggravated by a higher intake of total protein. The effects of diets with different proportions of protein and carbohydrate on diabetic retinopathy in db mice, a type-2 diabetes animal model, were examined, as well as diabetic nephropathy. Materials and Methods: Control and db mice at 5 weeks of age were fed the diets (% energy of protein/carbohydrate/fat; L-diet: 12/71/17; H-diet: 24/59/17) under ad libitum conditions and pair-feeding conditions for 6 weeks, respectively. Results: Mice fed the H-diet showed significantly greater retinal thickness by optical coherence tomography, and lower mRNA levels of angiotensinogen. Comparing combinations of diets and genotypes, db-H mice showed significantly higher mRNA levels of angiotensin-converting enzyme, advanced glycosylation end product-specific receptor, and cluster of differentiation molecule 11b (a microglial marker) than db-L mice. Conclusion: Dietary protein and carbohydrate proportions influenced retinal manifestations, including retinal thickness and gene expression in control and diabetic mice. ER -