RT Journal Article
SR Electronic
T1 Tissue-directed Implantation Using Ultrasound Visualization for Development of Biologically Relevant Metastatic Tumor Xenografts
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 779
OP 791
VO 31
IS 5
A1 RAELENE A. VAN NOORD
A1 TINA THOMAS
A1 MELANIE KROOK
A1 SAHITI CHUKKAPALLI
A1 MARK J. HOENERHOFF
A1 JONATHAN R. DILLMAN
A1 ELIZABETH R. LAWLOR
A1 VALERIE P. OPIPARI
A1 ERIKA A. NEWMAN
YR 2017
UL http://iv.iiarjournals.org/content/31/5/779.abstract
AB Background: Advances in cancer therapeutics depend on reliable in vivo model systems. To develop biologically relevant xenografts, ultrasound was utilized for tissue-directed implantation of neuroblastoma (NB) cell line and patient-derived tumors in the adrenal gland, and for renal subcapsular engraftment of Ewing's sarcoma (ES). Materials and Methods: NB xenografts were established by direct adrenal injection of luciferase-transfected NB cell lines (IMR32, SH-SY5Y, SK-N-BE2) or NB patient-derived tumor cells (UMNBL001, UMNBL002). ES xenografts were established by renal subcapsular injection of TC32, A673, CHLA-25, or A4573 cells. Progression was monitored by in vivo imaging. Results: Tumors progressed to local disease with metastasis evident by 5 weeks. Metastatic sites included cortical bone, lung, liver, and lymph nodes. Xenografted tumors retained immunochemical features of the original cancer. Conclusion: Human NB adrenal xenografts, including two patient-derived orthotopic, and ES renal subcapsular xenografts were established by ultrasound without open surgery. Tissue-directed implantation is an effective technique for developing metastatic preclinical models.