TY - JOUR T1 - Tissue-directed Implantation Using Ultrasound Visualization for Development of Biologically Relevant Metastatic Tumor Xenografts JF - In Vivo JO - In Vivo SP - 779 LP - 791 VL - 31 IS - 5 AU - RAELENE A. VAN NOORD AU - TINA THOMAS AU - MELANIE KROOK AU - SAHITI CHUKKAPALLI AU - MARK J. HOENERHOFF AU - JONATHAN R. DILLMAN AU - ELIZABETH R. LAWLOR AU - VALERIE P. OPIPARI AU - ERIKA A. NEWMAN Y1 - 2017/09/01 UR - http://iv.iiarjournals.org/content/31/5/779.abstract N2 - Background: Advances in cancer therapeutics depend on reliable in vivo model systems. To develop biologically relevant xenografts, ultrasound was utilized for tissue-directed implantation of neuroblastoma (NB) cell line and patient-derived tumors in the adrenal gland, and for renal subcapsular engraftment of Ewing's sarcoma (ES). Materials and Methods: NB xenografts were established by direct adrenal injection of luciferase-transfected NB cell lines (IMR32, SH-SY5Y, SK-N-BE2) or NB patient-derived tumor cells (UMNBL001, UMNBL002). ES xenografts were established by renal subcapsular injection of TC32, A673, CHLA-25, or A4573 cells. Progression was monitored by in vivo imaging. Results: Tumors progressed to local disease with metastasis evident by 5 weeks. Metastatic sites included cortical bone, lung, liver, and lymph nodes. Xenografted tumors retained immunochemical features of the original cancer. Conclusion: Human NB adrenal xenografts, including two patient-derived orthotopic, and ES renal subcapsular xenografts were established by ultrasound without open surgery. Tissue-directed implantation is an effective technique for developing metastatic preclinical models. ER -