PT  - JOURNAL ARTICLE
AU  - IKITIMUR-ARMUTAK, ELIF I.
AU  - ULUKAYA, ENGIN
AU  - GUREL-GUREVIN, EBRU
AU  - YAYLIM, ILHAN
AU  - ISBILEN-BASOK, BANU
AU  - SENNAZLI, GULBIN
AU  - YUZBASIOGLU-OZTURK, GULAY
AU  - SONMEZ, KIVILCIM
AU  - CELIK, FARUK
AU  - KUCUKHUSEYIN, OZLEM
AU  - KORKMAZ, GURBET
AU  - YILMAZ, VEYSEL T.
AU  - ZEYBEK, SAKIR UMIT
TI  - Apoptosis-inducing Effect of a Palladium(II) Complex-[PdCl(terpy)](sac).2H2O] on Ehrlich Ascites Carcinoma (EAC) in Mice
DP  - 2016 Jul 01
TA  - In Vivo
PG  - 457--464
VI  - 30
IP  - 4
4099  - http://iv.iiarjournals.org/content/30/4/457.short
4100  - http://iv.iiarjournals.org/content/30/4/457.full
SO  - In Vivo2016 Jul 01; 30
AB  - Background/Aim: New compounds for cancer treatment are needed due to persistenly unsatisfactory management of cancer. [PdCl(terpy)](sac)·2H2O] (sac=saccharinate, and terpy=2,2’:6’,2”-terpyridine) is a compound synthesized for this purpose. We investigated its anti-proliferative and pro-apoptotic effects on Ehrlich Ascites Carcinoma (EAC) in vivo. Materials and Methods: 42 Balb-c female mice were subcutaneously (s.c.) injected with EAC cells (1st day) and then randomly divided into 5 groups: control (0.9% NaCl), complex (2 mg/kg), complex (3 mg/kg) cisplatin (4 mg/kg) and paclitaxel (12.5 mg/kg). On the 5th and 12th day animals were drug administrated. At 14th day, animals were sacrificed. Expression of cell death and/or cell cycle-related markers (Bcl-2, Bax, active caspase-3, p53, PCNA) and apoptosis were investigated immunohisto-chemically. Survival-related markers (Akt, GSK-3β, IGF-1R, IR, IRS-1, p70S6K, PRAS40) were evaluated by luminex analysis. Results: Expression of p53, PCNA, Bcl-2 was found decreased (p<0.001) and that of active caspase-3, Bax, and apoptotic cells was found increased (p<0.001) in all groups. The survival-related markers did not show any statistical difference in complex groups. Conclusion: The Pd(II)-complex seems to have a strong anticancer activity on EAC by inducing apoptosis via both suppression of proliferation and activation of apoptosis in vivo, similar to the effects of cisplatin and paclitaxel.