RT Journal Article
SR Electronic
T1 Quest for Cells Responsible for Age-related Increase of Salivary Glycine and Proline
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 91
OP 97
VO 30
IS 2
A1 SHUNSUKE HINO
A1 AKIRA NISHIYAMA
A1 TOMOHIKO MATSUTA
A1 NORIO HORIE
A1 TETSUO SHIMOYAMA
A1 SHOJI TANAKA
A1 HIROSHI SAKAGAMI
YR 2016
UL http://iv.iiarjournals.org/content/30/2/91.abstract
AB We have previously reported that salivary glycine and proline levels are increased to nearly butanoate level in elderly people. In order to identify the source of glycine and proline, we performed high-performance liquid chromatography analysis of amino acid production to a total of seven oral cells before and after stimulation with inflammation inducers. We found that production of amino acids (per a given number of cells) by normal oral mesenchymal cells (gingival fibroblast, pulp cell, periodontal ligament fibroblast) was approximately three-fold that of oral squamous cell carcinoma cell lines (HSC-2, HSC-3, HSC-4, Ca9-22), and that production of glycine and especially proline by all these seven cells was much lower than that of glutamine and glutamic acid. Treatment of three oral mesenchymal cells with interleukin (IL)-1β or lipopoly-saccharide (LPS) reproducibly increased the production of glutamic acid and glutamine, but not that of glycine and proline. Glycine and proline only marginally stimulated the IL-8 production by IL-1β-stimulated gingival fibroblast, whereas glycine dose-dependently inhibited the nitric oxide production by lipopolysaccharide-stimulated mouse macrophage-like RAW264.7 cells. These data demonstrated that normal oral mesenchymal cells are not the major source of glycine and proline that accumulates in the saliva of aged people, suggesting the involvement of the deregulation of collagen metabolism during aging.