TY - JOUR T1 - Inhibition of Cell Growth of the Prostate Cancer Cell Model LNCaP by Cold Atmospheric Plasma JF - In Vivo JO - In Vivo SP - 611 LP - 616 VL - 29 IS - 5 AU - MARTIN WEISS AU - DENIS GÜMBEL AU - NADINE GELBRICH AU - LARS-OVE BRANDENBURG AU - ROBERT MANDELKOW AU - UWE ZIMMERMANN AU - PATRICK ZIEGLER AU - MARTIN BURCHARDT AU - MATTHIAS B. STOPE Y1 - 2015/09/01 UR - http://iv.iiarjournals.org/content/29/5/611.abstract N2 - Backround/Aim: Physical plasmas are ionized gases containing several biologically-reactive factors that yet exert their anti-microbial and anti-proliferative effects in fields of surface sterilisation, de-contamination and wound healing. Materials and Methods: Cold atmospheric plasma (CAP) was generated via the atmospheric pressure plasma jet kINPen09. Apoptotic effects of CAP treatment on the human epithelial prostate cancer cell line LNCaP as a cell culture model for malignant tumor tissue was analyzed by cell counting, western blot and quantitative reverse transcription-polymerase chain reaction analysis. Results: LNCaP cells exhibited significantly reduced cell growth following CAP treatment. We show that most probably the induction of apoptosis is the terminus of CAP treatment illustrated by the pro-apoptotic modulation of p53, p21, caspase-3, Bax, and survivin, as well as morphological changes of cell architecture. Conclusion: Our in vitro study offers first indicatory results for molecular response mechanisms after CAP treatment in a suitable LNCaP cell model. ER -