RT Journal Article
SR Electronic
T1 Hypoacetylation in Association with Histone 3 Modulation in Human Hepatocellular Carcinoma
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 237
OP 242
VO 29
IS 2
A1 CHIUNG-CHI CHENG
A1 YI-HSIANG LIU
A1 YEN-CHANG CLARK LAI
A1 YUNG-HSIANG HSU
A1 WEI-TING CHAO
A1 YIH-SHYONG LAI
YR 2015
UL http://iv.iiarjournals.org/content/29/2/237.abstract
AB Background: Aberrant histone deacetylase expression may cause imbalance between acetylation and deacetylation of histone and play roles in tumor transformation. We found that histone 3 was modulated in human hepatocellular carcinoma. We determined if histone 3 modulation is related to the aberrant expression of histone deacetylase. Materials and Methods: We analyzed human liver and hepatocellular carcinoma tissues and fibroblast and fibrosarcoma cell lines for the expression of histone 3, histone deacetylase 1 and acetylated histone 3 using immunohistochemistry, western blot and immunofluorescence. Results: Histone deacetylase 1 and histone 3 were more strongly detected in hepatocellular carcinoma tissue and fibrosarcoma cells than in liver tissues and fibroblast cells, respectively. However, acetylated histone 3 was more strongly expressed in normal liver and fibroblast cells and less expressed in hepatocellular carcinoma and fibrosarcoma cells. Conclusion: Histone deacetylase 1 overexpression and hypoacetylation of histone 3 might play critical roles in the modulation of histone 3 in human hepatocellular carcinoma.