TY - JOUR T1 - Hypoacetylation in Association with Histone 3 Modulation in Human Hepatocellular Carcinoma JF - In Vivo JO - In Vivo SP - 237 LP - 242 VL - 29 IS - 2 AU - CHIUNG-CHI CHENG AU - YI-HSIANG LIU AU - YEN-CHANG CLARK LAI AU - YUNG-HSIANG HSU AU - WEI-TING CHAO AU - YIH-SHYONG LAI Y1 - 2015/03/01 UR - http://iv.iiarjournals.org/content/29/2/237.abstract N2 - Background: Aberrant histone deacetylase expression may cause imbalance between acetylation and deacetylation of histone and play roles in tumor transformation. We found that histone 3 was modulated in human hepatocellular carcinoma. We determined if histone 3 modulation is related to the aberrant expression of histone deacetylase. Materials and Methods: We analyzed human liver and hepatocellular carcinoma tissues and fibroblast and fibrosarcoma cell lines for the expression of histone 3, histone deacetylase 1 and acetylated histone 3 using immunohistochemistry, western blot and immunofluorescence. Results: Histone deacetylase 1 and histone 3 were more strongly detected in hepatocellular carcinoma tissue and fibrosarcoma cells than in liver tissues and fibroblast cells, respectively. However, acetylated histone 3 was more strongly expressed in normal liver and fibroblast cells and less expressed in hepatocellular carcinoma and fibrosarcoma cells. Conclusion: Histone deacetylase 1 overexpression and hypoacetylation of histone 3 might play critical roles in the modulation of histone 3 in human hepatocellular carcinoma. ER -