RT Journal Article
SR Electronic
T1 Crude Extract of <em>Polygonum Cuspidatum</em> Promotes Immune Responses in Leukemic Mice Through Enhancing Phagocytosis of Macrophage and Natural Killer Cell Activities <em>In Vivo</em>
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 255
OP 261
VO 29
IS 2
A1 FU-SHIN CHUEH
A1 JEN-JYH LIN
A1 JING-PIN LIN
A1 FU-SHUN YU
A1 JU-HWA LIN
A1 YI-SHIH MA
A1 YI-PING HUANG
A1 JIN-CHERNG LIEN
A1 JING-GUNG CHUNG
YR 2015
UL http://iv.iiarjournals.org/content/29/2/255.abstract
AB Polygonum cuspidatum is a traditional Chinese herbal medicine used in the treatment of various diseases. In the present study, we investigated whether the crude extract of Polygonum cuspidatum (CEPC) could affect immune responses of murine leukemia cells in vivo. Normal BALB/c mice were i.p. injected with WEHI-3 cells to generate leukemic mice and then were treated orally with CEPC at 0, 50, 100 and 200 mg/kg for three weeks. Animals were weighed and blood, liver, spleen samples were collected for further analyses. Results indicated that CEPC did not significantly affect the body and liver weight of animals, but reduced the weight of spleen when compared to control groups. Flow cytometric assay demonstrated that CEPC increased the percentage of CD3- (T-cell marker) and CD19- (B-cell marker) positive cells, but reduced that of CD11b-positive ones (monocytes). However, it did not significantly affect the proportion of Mac-3-positive cells (macrophages), compared to control groups. Results indicated that CEPC promoted phagocytosis by macrophages from blood samples at all examined doses but did not affect that of macrophages from the peritoneal cavity. CEPC also promoted natural killer cell activity of splenocytes at 200 mg/kg of CEPC. CEPC promoted B-cell proliferation at 200 mg/kg treatment when cells were stimulated with lipopolysaccharides but did not promote T-cell proliferation at three doses of CEPC treatment on concanavalin A stimulation.