@article {GARCIA-CONTRERAS895, author = {RENE GARCIA-CONTRERAS and ROGELIO J. SCOUGALL-VILCHIS and ROSALIA CONTRERAS-BULNES and YUMIKO KANDA and HIROSHI NAKAJIMA and HIROSHI SAKAGAMI}, title = {Effects of TiO2 Nano Glass Ionomer Cements Against Normal and Cancer Oral Cells}, volume = {28}, number = {5}, pages = {895--907}, year = {2014}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Incorporation of nanoparticles (NPs) into the glass ionomer cements (GICs) is known to improve their mechanical and antibacterial properties. The present study aimed to investigate the possible cytotoxicity and pro-inflammation effect of three different powdered GICs (base, core build and restorative) prepared with and without titanium dioxide (TiO2) nanoparticles. Materials and Methods: Each GIC was blended with TiO2 nanopowder, anatase phase, particle size \<25 nm at 3\% and 5\% (w/w), and the GIC blocks of cements were prepared in a metal mold. The GICs/TiO2 nanoparticles cements were smashed up with a mortar and pestle to a fine powder, and then subjected to the sterilization by autoclaving. Human oral squamous cell carcinoma cell lines (HCS-2, HSC-3, HSC-4, Ca9-22) and human normal oral cells [gingival fibroblast (HGF), pulp (HPC) and periodontal ligament fibroblast (HPLF)] were incubated with different concentrations of GICs in the presence or absence of TiO2 nanoparticles, and the viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Prostaglandin E2 was quantified by enzyme-linked immunosorbent assay (ELISA). Changes in fine cell structure were assessed by transmission electron microscopy. Results: Cancer cells exhibited moderate cytotoxicity after 48 h of incubation, regardless of the type of GIC and the presence or absence of TiO2 NPs. GICs induced much lower cytotoxicity against normal cells, but induced prostaglandin E2 production, in a synergistic wanner with interleukin-1β. Conclusion: The present study shows acceptable to moderate biocompatibility of GICs impregnated with TiO2 nanoparticles, as well as its pro-inflammatory effects at higher concentrations.}, issn = {0258-851X}, URL = {https://iv.iiarjournals.org/content/28/5/895}, eprint = {https://iv.iiarjournals.org/content/28/5/895.full.pdf}, journal = {In Vivo} }