RT Journal Article
SR Electronic
T1 Bone Substitutes Enhance Osteogenic Differentiation of Mesenchymal Stem Cells in Three-dimensional Scaffolds
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 733
OP 739
VO 28
IS 5
A1 HANKEN, HENNING
A1 ALPERS, JANNA
A1 BÖBEL, MELANIE
A1 WÖLTJE, MICHAEL
A1 HARTJEN, PHILIP
A1 FRIEDRICH, REINHARD E.
A1 JUNG, OLE
A1 RENDENBACH, CARSTEN
A1 GRÖBE, ALEXANDER
A1 HEILAND, MAX
A1 AL-DAM, AHMAD
A1 EICHHORN, WOLFGANG
A1 SMEETS, RALF
YR 2014
UL http://iv.iiarjournals.org/content/28/5/733.abstract
AB Aim: The present study aimed to find bone substitutes to enhance osteogenic differentiation of mesenchymal stem cells in three-dimensional scaffolds in the absence of dexamethasone. Materials and Methods: Seven commercial bone substitutes were added to a three-dimensional fibrin-matrix containing rat mesenchymal stem cells in a biocompatible poly-L-lactic-acid mesh. Cell viability, cytotoxicity and alkaline phosphatase activity were followed for three weeks. Expression of bone markers was examined by qualitative evaluation of corresponding transcripts. Results: Six out of the seven bone derivatives exhibited an osteogenic-enhancing effect. Conclusion: The osteogenic-enhancing effect of the evaluated bone substitutes suggests their potential clinical application for preparation of autologous bone replacement material in three-dimensional carriers.