PT  - JOURNAL ARTICLE
AU  - HANKEN, HENNING
AU  - ALPERS, JANNA
AU  - BÖBEL, MELANIE
AU  - WÖLTJE, MICHAEL
AU  - HARTJEN, PHILIP
AU  - FRIEDRICH, REINHARD E.
AU  - JUNG, OLE
AU  - RENDENBACH, CARSTEN
AU  - GRÖBE, ALEXANDER
AU  - HEILAND, MAX
AU  - AL-DAM, AHMAD
AU  - EICHHORN, WOLFGANG
AU  - SMEETS, RALF
TI  - Bone Substitutes Enhance Osteogenic Differentiation of Mesenchymal Stem Cells in Three-dimensional Scaffolds
DP  - 2014 Sep 01
TA  - In Vivo
PG  - 733--739
VI  - 28
IP  - 5
4099  - http://iv.iiarjournals.org/content/28/5/733.short
4100  - http://iv.iiarjournals.org/content/28/5/733.full
SO  - In Vivo2014 Sep 01; 28
AB  - Aim: The present study aimed to find bone substitutes to enhance osteogenic differentiation of mesenchymal stem cells in three-dimensional scaffolds in the absence of dexamethasone. Materials and Methods: Seven commercial bone substitutes were added to a three-dimensional fibrin-matrix containing rat mesenchymal stem cells in a biocompatible poly-L-lactic-acid mesh. Cell viability, cytotoxicity and alkaline phosphatase activity were followed for three weeks. Expression of bone markers was examined by qualitative evaluation of corresponding transcripts. Results: Six out of the seven bone derivatives exhibited an osteogenic-enhancing effect. Conclusion: The osteogenic-enhancing effect of the evaluated bone substitutes suggests their potential clinical application for preparation of autologous bone replacement material in three-dimensional carriers.