TY - JOUR T1 - Improved Longevity of Hematopoiesis in Long-term Bone Marrow Cultures and Reduced Irradiation-induced Pulmonary Fibrosis in Toll-like Receptor-4 Deletion Recombinant-Negative Mice JF - In Vivo JO - In Vivo SP - 441 LP - 448 VL - 28 IS - 4 AU - BYUNG HAN RHIEU AU - MICHAEL W. EPPERLY AU - SHAONAN CAO AU - JULIE GOFF AU - DONNA SHIELDS AU - DARCY FRANICOLA AU - HONG WANG AU - JOEL S. GREENBERGER Y1 - 2014/07/01 UR - http://iv.iiarjournals.org/content/28/4/441.abstract N2 - Aim: We measured long-term hematopoiesis in continuous bone marrow cultures derived from Toll-like receptor-4 (Tlr4−/−)(C57BL/6J) mice. Materials and Methods: We measured hematopoiesis in vitro over 27 weeks in long-term bone marrow cultures from Tlr4−/− and control mice, and irradiation-induced pulmonary fibrosis in mice irradiated to 20 Gy to the thorax. Results: There was a significant increase in the duration of hematopoiesis in long-term bone marrow cultures from Tlr4−/− mice in production of total non-adherent cells and day 7 and day 14 multi-lineage colony-forming cells. The histology of bone marrow hematopoietic and stromal cell lines was indistinguishable between different mouse strains. There was no detectable late irradiation pulmonary fibrosis in Tlr4−/− mice. Conclusion: Homozygous deletion of both alleles of Tlr4, encoding for an inflammatory mediator receptor, improves the duration of hematopoiesis in vitro and reduces irradiation-induced lung fibrosis. ER -