RT Journal Article
SR Electronic
T1 Effects of the Bifunctional Sulfoxide MMS350, a Radiation Mitigator, on Hematopoiesis in Long-term Bone Marrow Cultures and on Radioresistance of Marrow Stromal Cell Lines
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 457
OP 465
VO 28
IS 4
A1 ASHWIN SHINDE
A1 MICHAEL W. EPPERLY
A1 SHAONAN CAO
A1 DARCY FRANICOLA
A1 DONNA SHIELDS
A1 HONG WANG
A1 PETER WIPF
A1 MELISSA M. SPRACHMAN
A1 JOEL S. GREENBERGER
YR 2014
UL http://iv.iiarjournals.org/content/28/4/457.abstract
AB The ionizing irradiation mitigator MMS350 prolongs survival of mice treated with total-body irradiation and prevents radiation-induced pulmonary fibrosis when added to drinking water at day 100 after thoracic irradiation. The effects of MMS350 on hematopoiesis in long-term bone marrow culture and on the radiobiology of derived bone marrow stromal cell lines were tested. Long-term bone marrow cultures were established from C57BL/6NTac mice and maintained in a high-humidity incubator, with 7% CO2 and the addition of 100 μM MMS350 at the weekly media change. Over 10 weeks in culture, MMS350 had no significant effect on maintenance of hematopoietic stem cell production, or on nonadherent cells or colony-forming units of hematopoietic progenitor cells. Stromal cell lines derived from non MMS350-treated long-term cultures or control stromal cells treated with MMS350 were radioresistant in the clonogenic survival curve assay. MMS350 is a non-toxic, highly water-soluble radiation mitigator that exhibits radioprotective effects on bone marrow stromal cells.