RT Journal Article SR Electronic T1 Alpha-phellandrene, a Natural Active Monoterpene, Influences a Murine WEHI-3 Leukemia Model In Vivo by Enhancing Macrophague Phagocytosis and Natural Killer Cell Activity JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 583 OP 588 VO 28 IS 4 A1 JEN-JYH LIN A1 KUNG-WEN LU A1 YI-SHIH MA A1 NOU-YING TANG A1 PING-PING WU A1 CHIH-CHUNG WU A1 HSU-FENG LU A1 JAUNG-GENG LIN A1 JING-GUNG CHUNG YR 2014 UL http://iv.iiarjournals.org/content/28/4/583.abstract AB α-phellandrene (α-PA), a cyclic monoterpene, is a natural compound reported to promote immune responses in normal BALB/c mice. The effects of α-PA on immune responses in a leukemia mouse model were examined. Mice were injected with mouse leukemia WEHI-3 cells and subsequently treated orally with or without α-PA (0, 25 and 50 mg/kg) and olive oil as positive control for two weeks. Leukocytes and splenocytes were isolated and cell markers for CD3, CD19, CD11b and Mac-3, phagocytosis and natural killer cell cytoxicity effects were analyzed by flow cytometry. α-PA increased the percentage of CD3 (T-cell marker), CD19 (B-cell marker) and MAC3 (macrophages) markers but reduced the percentage of CD11b (monocytes) cell surface markers. α-PA (25 and 50 mg/kg) increased phagocytosis of macrophages from blood samples and treatment promoted natural killer cell activity at 25 mg/kg from splenocytes. α-PA at 25 mg/kg also increased B- and T-cell proliferation.