PT  - JOURNAL ARTICLE
AU  - JEN-JYH LIN
AU  - KUNG-WEN LU
AU  - YI-SHIH MA
AU  - NOU-YING TANG
AU  - PING-PING WU
AU  - CHIH-CHUNG WU
AU  - HSU-FENG LU
AU  - JAUNG-GENG LIN
AU  - JING-GUNG CHUNG
TI  - Alpha-phellandrene, a Natural Active Monoterpene, Influences a Murine WEHI-3 Leukemia Model <em>In Vivo</em> by Enhancing Macrophague Phagocytosis and Natural Killer Cell Activity
DP  - 2014 Jul 01
TA  - In Vivo
PG  - 583--588
VI  - 28
IP  - 4
4099  - http://iv.iiarjournals.org/content/28/4/583.short
4100  - http://iv.iiarjournals.org/content/28/4/583.full
SO  - In Vivo2014 Jul 01; 28
AB  - α-phellandrene (α-PA), a cyclic monoterpene, is a natural compound reported to promote immune responses in normal BALB/c mice. The effects of α-PA on immune responses in a leukemia mouse model were examined. Mice were injected with mouse leukemia WEHI-3 cells and subsequently treated orally with or without α-PA (0, 25 and 50 mg/kg) and olive oil as positive control for two weeks. Leukocytes and splenocytes were isolated and cell markers for CD3, CD19, CD11b and Mac-3, phagocytosis and natural killer cell cytoxicity effects were analyzed by flow cytometry. α-PA increased the percentage of CD3 (T-cell marker), CD19 (B-cell marker) and MAC3 (macrophages) markers but reduced the percentage of CD11b (monocytes) cell surface markers. α-PA (25 and 50 mg/kg) increased phagocytosis of macrophages from blood samples and treatment promoted natural killer cell activity at 25 mg/kg from splenocytes. α-PA at 25 mg/kg also increased B- and T-cell proliferation.