TY - JOUR T1 - Effects of TiO<sub>2</sub> Nanoparticles on Cytotoxic Action of Chemotherapeutic Drugs Against a Human Oral Squamous Cell Carcinoma Cell Line JF - In Vivo JO - In Vivo SP - 209 LP - 215 VL - 28 IS - 2 AU - RENE GARCIA-CONTRERAS AU - ROGELIO J. SCOUGALL-VILCHIS AU - ROSALIA CONTRERAS-BULNES AU - YOSHIAKI ANDO AU - YUMIKO KANDA AU - YASUSHI HIBINO AU - HIROSHI NAKAJIMA AU - HIROSHI SAKAGAMI Y1 - 2014/03/01 UR - http://iv.iiarjournals.org/content/28/2/209.abstract N2 - Background. Despite the rapid development of nanotechnology, the biological significance of TiO2 nanoparticles (NPs), possibly released from dental materials, is not well-understood. We investigated the effect of TiO2 NPs on the sensitivity of human oral squamous cell carcinoma (OSCC) cell line (HSC-2) to five popular chemotherapeutic agents. Materials and Methods. Viable cell number was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The aggregation and cellular uptake of TiO2 NPs were assessed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. Adsorption of TiO2 NPs to anticancer drugs was assessed by the antitumor activity recovered from the TiO2 NP-free supernatant. Results: When mixed with culture medium, TiO2 NPs instantly aggregated, and some particles were incorporated into the cells, exclusively in the vacuoles. TiO2 NPs showed no cytotoxicity nor hormetic growth stimulation at lower concentrations. Doxorubicin, melphalan, 5-fluorouracil and gefitinib were cytotoxic, whereas docetaxel was cytostatic with or without TiO2 NPs. TiO2 NPs, at wide concentration ranges (0.2-3.2 mM), did not significantly affect the adsorption of NPs to any of these anticancer drugs, nor affected their cytotoxic or cytostatic activity. Conclusion: This experimental study demonstrated for the first time that TiO2 NP do not affect the antitumor potential of chemotherapeutic agents against the HSC-2 OSCC cell line. ER -