RT Journal Article
SR Electronic
T1 Changes of Metabolic Profiles in an Oral Squamous Cell Carcinoma Cell Line Induced by Eugenol
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 233
OP 243
VO 27
IS 2
A1 TEHO KOH
A1 YUKIO MURAKAMI
A1 SHOJI TANAKA
A1 MAMORU MACHINO
A1 HIROMI ONUMA
A1 MIKU KANEKO
A1 MASAHIRO SUGIMOTO
A1 TOMOYOSHI SOGA
A1 MASARU TOMITA
A1 HIROSHI SAKAGAMI
YR 2013
UL http://iv.iiarjournals.org/content/27/2/233.abstract
AB Background: We have recently reported that eugenol exerted indiscriminate cytotoxicity towards normal oral cells and oral squamous cell carcinoma (OSCC) cell lines without induction of apoptosis markers. In order to investigate the underlying mechanisms of cytotoxicity induction, we investigated the effect of short-term treatment with eugenol on the metabolic profiles of a human OSCC cell line (HSC-2). Materials and Methods: The viable cell number was determined by direct cell counting with a hemocytometer after trypsinization. After washing with 5% D-mannitol solution (found to retain the highest amounts of intracellular metabolites among several washing conditions), cellular metabolites were extracted with methanol with internal markers and then subjected to metabolomic analysis. Results: Cytotoxic concentrations of eugenol induced the reduction of ATP utilization (assessed by a significant reduction of the AMP/ATP and ADP/ATP ratio), of oxidative stress (assessed by the increase in oxidized form of glutathione, cysteine-glutathione disulfide and methionine sulfoxide), and an increase in the polyamines and glycolytic metabolites. Conclusion: The metabolic changes observed in this study suggest the induction of non-apoptotic cell death by eugenol.