RT Journal Article
SR Electronic
T1 Very Early Effect of DMBA and MNU on MicroRNA Expression
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 113
OP 117
VO 27
IS 1
A1 JUHÁSZ, KRISZTINA
A1 GOMBOS, KATALIN
A1 SZIRMAI, MÓNIKA
A1 GŐCZE, KATALIN
A1 WOLHER, VERONIKA
A1 RÉVÉSZ, PÉTER
A1 MAGDA, INGRID
A1 SEBESTYÉN, ANDOR
A1 NÉMETH, ÁRPÁD
A1 EMBER, ISTVÁN
YR 2013
UL http://iv.iiarjournals.org/content/27/1/113.abstract
AB Background: microRNA expression profile analysis provides evaluation of the early stages of carcinogenesis. This study focuses on early alteration of miRNA expression after treatment with different carcinogens. Materials and Methods: Mice were intraperitoneally injected with one dose of 7,12-dimethylbenz(α)anthracene (DMBA) and N-methyl-N-nitrosourea (MNU). The expression of miRNAs were analyzed 3 and 6 hours after the treatment, using quantitative real-time-polymerase chain reaction. Results: Underexpression of miR-34a and miR-155 were detected in the liver, spleen and kidneys at 3 and 6 hours after MNU treatment. In the lungs and kidneys, the expression of miR-21 was significantly elevated 6 hours after DMBA treatment, while in the liver, MNU induced higher expression levels of miR-21 at 3 and 6 hours compared to treatment with DMBA. Conclusion: The different response of miRNAs to carcinogens emphasizes their possible role as potential epidemiological biomarkers in early phases of environmental tumorigenesis.