PT  - JOURNAL ARTICLE
AU  - JUHÁSZ, KRISZTINA
AU  - GOMBOS, KATALIN
AU  - SZIRMAI, MÓNIKA
AU  - GŐCZE, KATALIN
AU  - WOLHER, VERONIKA
AU  - RÉVÉSZ, PÉTER
AU  - MAGDA, INGRID
AU  - SEBESTYÉN, ANDOR
AU  - NÉMETH, ÁRPÁD
AU  - EMBER, ISTVÁN
TI  - Very Early Effect of DMBA and MNU on MicroRNA Expression
DP  - 2013 Jan 01
TA  - In Vivo
PG  - 113--117
VI  - 27
IP  - 1
4099  - http://iv.iiarjournals.org/content/27/1/113.short
4100  - http://iv.iiarjournals.org/content/27/1/113.full
SO  - In Vivo2013 Jan 01; 27
AB  - Background: microRNA expression profile analysis provides evaluation of the early stages of carcinogenesis. This study focuses on early alteration of miRNA expression after treatment with different carcinogens. Materials and Methods: Mice were intraperitoneally injected with one dose of 7,12-dimethylbenz(α)anthracene (DMBA) and N-methyl-N-nitrosourea (MNU). The expression of miRNAs were analyzed 3 and 6 hours after the treatment, using quantitative real-time-polymerase chain reaction. Results: Underexpression of miR-34a and miR-155 were detected in the liver, spleen and kidneys at 3 and 6 hours after MNU treatment. In the lungs and kidneys, the expression of miR-21 was significantly elevated 6 hours after DMBA treatment, while in the liver, MNU induced higher expression levels of miR-21 at 3 and 6 hours compared to treatment with DMBA. Conclusion: The different response of miRNAs to carcinogens emphasizes their possible role as potential epidemiological biomarkers in early phases of environmental tumorigenesis.