RT Journal Article
SR Electronic
T1 Anti-UV/HIV Activity of Kampo Medicines and Constituent Plant Extracts
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 1007
OP 1013
VO 26
IS 6
A1 KATO, TAKAO
A1 HORIE, NORIO
A1 MATSUTA, TOMOHIKO
A1 NAOKI, UMEMURA
A1 SHIMOYAMA, TETSUO
A1 KANEKO, TADAYOSHI
A1 KANAMOTO, TAISEI
A1 TERAKUBO, SHIGEMI
A1 NAKASHIMA, HIDEKI
A1 KUSAMA, KAORU
A1 SAKAGAMI, HIROSHI
YR 2012
UL http://iv.iiarjournals.org/content/26/6/1007.abstract
AB Aim: In order to search for new biological activities of Kampo medicines and their constituent plant extracts, we investigated whether they protect the cells from the cytotoxicity induced by UV irradiation and human immunodeficiency virus (HIV) infection. Materials and Methods: Anti-UV/HIV activity (SI value) was evaluated as the ratio of the CC50 (concentration that reduced the viable cell number by 50%) to the EC50 (the concentration that increased the viability of UV-irradiated or HIV-infected cells to 50%): SI=CC50/EC50. The content of glycyrrhizin in each sample was determined by high performance liquid chromatography (HPLC). Caspase-3/-7 activity was assayed by cleavage of poly ADP ribose polymerase using western blot analysis. Results: Among 25 plant extracts, Gardenia fruit had the highest anti-UV activity (SI≥8.0), followed by Glycyrrhiza (SI=4.3), Coptis rhizoma (SI=1.5), Cimicifuga rhizoma (SI>1.4), Saposhnikovia root (SI>1.3) and Japanese Gentian (SI>1.1). Among ten Kampo medicines, Unseiin and Hangesyashinto (SI>4.9) had the highest anti-UV activity, followed by Shosaikoto (SI>4.3), Saireito (SI>3.4), Rikkosan (SI>1.2) and Kikyoto (SI=1.1). Glycyrrhiza inhibited UV-induced caspase-3/-7 activation. Only Polyporus sclerotium (SI>4.4), Gardenia fruit (SI>2.7), Atractylodes lancea rhizoma (SI>1.9), Cnidium rhizoma (SI>1.5) and Japanese Angelica root (SI>1.1) exhibited some anti-HIV activity. There was no apparent correlation of their anti-UV/HIV activity and content of glycyrrhizin, a major component of Glycyrrhiza, which exhibited much higher anti-UV activity (SI=20.6) and some anti-HIV activity (SI>2.0). Conclusion: The present study suggests the involvement of substances other than glycyrrhizin in the anti-UV/HIV activity of Kampo medicines and their constituent plant extracts.