TY - JOUR T1 - Amentoflavone Induces Cell-cycle Arrest and Apoptosis in MCF-7 Human Breast Cancer Cells <em>via</em> Mitochondria-dependent Pathway JF - In Vivo JO - In Vivo SP - 963 LP - 970 VL - 26 IS - 6 AU - JEN-SHENG PEI AU - CHIA-CHI LIU AU - YUAN-NIAN HSU AU - LI-LING LIN AU - SHOU-CHENG WANG AU - JING-GUNG CHUNG AU - DA-TIAN BAU AU - SONG-SHEI LIN Y1 - 2012/11/01 UR - http://iv.iiarjournals.org/content/26/6/963.abstract N2 - Amentoflavone, isolated from an ethyl acetate extract of the whole plant of Selaginella tamariscina, a traditional herb, may exhibit antitumor activity. The aim of this study was to investigate the anticancer mechanism(s) of amentoflavone, such as mitochondria-mediated apoptotic cell death, in typical breast cancer MCF-7 cells. Cells treated with amentoflavone exhibited a series of cellular alterations related to apoptosis, including DNA and nuclear fragmentation, and de-regulation of intracellular reactive oxygen species (ROS) and calcium. In addition, markers of mitochondrial-mediated apoptosis, including the reduction of mitochondrial inner-membrane potential, the release of cytochrome c from mitochondria, and activation of caspase 3, were observed. In conclusion, our results present, to our knowledge, the first evidence that amentoflavone induces apoptosis of MCF-7 breast cancer cells, and that this is closely related to mitochondrial dysfunction. Amentoflavone may be a potential therapeutic agent for breast cancer treatment. ER -