TY - JOUR T1 - Histology, Bioenergetics and Oxidative Stress in Mouse Liver Exposed to <em>N</em>-Diethylnitrosamine JF - In Vivo JO - In Vivo SP - 921 LP - 929 VL - 26 IS - 6 AU - NUNO PAULA SANTOS AU - IVO CONCEIÇÃO PEREIRA AU - MARIA JOÃO PIRES AU - CARLOS LOPES AU - RITA ANDRADE AU - MARIA MANUEL OLIVEIRA AU - AURA COLAÇO AU - FRANCISCO PEIXOTO AU - PAULA ALEXANDRA OLIVEIRA Y1 - 2012/11/01 UR - http://iv.iiarjournals.org/content/26/6/921.abstract N2 - Background: A mouse model in which N-diethylnitrosamine (DEN) induces Hepatocellular carcinoma (HCC) has histological and genetic resemblance to human tumours. Material and Methods: Male ICR mice were divided into control (n=10) and DEN-treated (n=10) groups. DEN was administered via intraperitoneal injection, once a week, for eight consecutive weeks. Animals were euthanized seven weeks after the last administration of DEN and their livers were collected. Plasma albumin, total bilirubin, alanine transaminase and aspartate aminotransferase activity were all measured and liver mitochondrial bioenergetics and oxidative stress were also evaluated. Results: Histologically, pre-neoplastic lesions were identified in the livers of mice from the DEN group. Total plasma bilirubin increased significantly in the group exposed to DEN and mitochondrial complex I and IV were significantly inhibited (p=0.0403 and p=0.0053, respectively). Conclusion: DEN induced changes in liver bioenergetics and antioxidant capacity towards reactive oxygen species, seven weeks after administration. At this stage, liver tissues in mice exposed to DEN still had the ability to counteract the oxidative effects of DEN by increasing the activity of antioxidant enzymes. ER -