PT - JOURNAL ARTICLE AU - SIVAPRIYA PONNURANGAM AU - FADEE G. MONDALEK AU - JANITA GOVIND AU - DHARMALINGAM SUBRAMANIAM AU - COURTNEY W. HOUCHEN AU - SHRIKANT ANANT AU - PANAYOTIS PANTAZIS AU - RAMA P. RAMANUJAM TI - Urine and Serum Analysis of Consumed Curcuminoids Using an IκB-Luciferase Surrogate Marker Assay DP - 2010 Nov 01 TA - In Vivo PG - 861--864 VI - 24 IP - 6 4099 - http://iv.iiarjournals.org/content/24/6/861.short 4100 - http://iv.iiarjournals.org/content/24/6/861.full SO - In Vivo2010 Nov 01; 24 AB - Background: Curcumin metabolites are detectable in body fluids such as serum and urine. We have developed a novel assay that can detect metabolites in such body fluids by measuring their effect on the nuclear factor kappa B/inhibitor of kappa B (NF-κB/IκB) pathway. Patients and Methods: Fifteen healthy individuals were enrolled in the study and randomly assigned to two groups: control group (five) and curcumin group (ten). The test group ingested 8 g of the curcuminoids (C3-Complex™) with 16 oz of bottled water. Blood and urine were collected at 0, 4, 8, and 24 h after ingestion. Degradation of the NF-κB/IκB complex was detected by the Genetic Expression and Measurement (GEM™) assay using HCT116 cells stably transfected with PGL3-IκB firefly luciferase. Results: Using our novel GEM assay, the five controls who had not taken curcumin were identified. Conclusion: The GEM assay is a very sensitive and accurate non-invasive assay that could be utilized to detect metabolites in body fluids. It could also serve as a tool to determine participants' compliance during clinical research studies.