RT Journal Article SR Electronic T1 Hormetic and Anti-radiation Effects of Tropolone-related Compounds JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 843 OP 851 VO 24 IS 6 A1 KAORI KANTOH A1 MANAMI ONO A1 YUKO NAKAMURA A1 YUKIO NAKAMURA A1 KEN HASHIMOTO A1 HIROSHI SAKAGAMI A1 HIDETSUGU WAKABAYASHI YR 2010 UL http://iv.iiarjournals.org/content/24/6/843.abstract AB We have previously investigated a total of 173 azulene-, tropolone- and azulenequinone-related compounds for their tumor-specificity and anti-inflammatory activity. In this study, we selected six compounds that showed tumor-specific cytotoxicity (referred to as group I compounds) and five compounds that inhibited nitric oxide production by activated macrophages (referred to as group II compounds) to investigate their possible hormetic and anti-radiation effects. We have established three oral normal cell type, human gingival fibroblast HGF-1, pulp cell HPC-1 and periodontal ligament fibroblast HPLF-1, from extracted teeth and periodontal tissue. These normal cells expressed p53 protein, regardless of the growth stage (either at growing or near confluent phase), more than oral squamous cell carcinoma cell line (HSC-2). Group I compounds slightly stimulated the growth of HPL-1 cells only at restricted durations and concentrations, but did not affect that of HGF-1 and HPC-1 cells, suggesting the minor hormetic effects displayed by these compounds. We established a new evaluation system for UV-induced cellular damage using an intact HSC-2 cell system in which sodium ascorbate (vitamin C) and gallic acid, but not N-acetyl-l-cysteine nor catalase, exerted protective effects. Three group I compounds and two group II compounds significantly protected the cells from UV-induced injury, suggesting their possible anti-UV effect.