TY - JOUR T1 - Transplanted Mesenchymal Stem Cells Can Inhibit the Three Developmental Stages of Murine Acute Graft-<em>versus</em>-Host Disease JF - In Vivo JO - In Vivo SP - 659 LP - 666 VL - 24 IS - 5 AU - HONG LI AU - ZIKUAN GUO AU - HENG ZHU AU - XIU SEN LI AU - XIAOXIA JIANG AU - HUIYU YAO AU - XIAOYAN WANG AU - LI LIAO AU - YUANLIN LIU AU - YING WU AU - YI ZHANG AU - NING MAO Y1 - 2010/09/01 UR - http://iv.iiarjournals.org/content/24/5/659.abstract N2 - Background: Recent data have demonstrated that mesenchymal stem cells (MSCs) have potent immune regulation capacity in vitro, enhancing their therapeutic appeal for their utilisation in the management of acute graft-versus-host disease (aGvHD). However, their immunoregulatory activity in vivo is largely unknown. Materials and Methods: Using murine compact bone-derived MSCs in an aGvHD model, the phenotypic status of splenocytes of aGvHD and aGvHD+MSC groups of mice were examined by flow cytometry. Results: MSC infusion decreased the expression of MHC-II and CD69 molecules on splenic CD11b+ cells of aGvHD mice, which resulted in decreased maturation of antigen-presenting cells. Moreover, the down-regulated ratio of CD3+CD69+ to CD3+ cells, leading to restrained early activation and effector T-cell formation, resulted in the enhancement of the absolute and relative number of splenic CD3+ cells by MSCs co-transfer. Conclusion: This study demonstrated that MSCs can inhibit the three developmental stages of aGvHD. ER -