PT  - JOURNAL ARTICLE
AU  - HANS-UDO KASPER
AU  - ELISABETH KONZE
AU  - MICHAEL KERN
AU  - DIRK LUDGER STIPPEL
TI  - CD95 and TNFα-induced Apoptosis in Liver Metastases of Colorectal Carcinoma
DP  - 2010 Sep 01
TA  - In Vivo
PG  - 653--657
VI  - 24
IP  - 5
4099  - http://iv.iiarjournals.org/content/24/5/653.short
4100  - http://iv.iiarjournals.org/content/24/5/653.full
SO  - In Vivo2010 Sep 01; 24
AB  - Background: Therapeutic options in patients with metastatic colorectal cancer are still limited. As apoptosis contributes to the overall sensitivity to radiotherapy or chemotherapy, a better understanding of the apoptotic process in metastatic tumour tissues is necessary. Materials and Methods: Precision cut tissue slices (PCTS) of three human liver metastases were used to investigate the effect of activating CD95 antibodies (concentrations: 0.1 μg/ml, 1 μg/ml and 1 μg/ml and 1 μg/ml actinomycin D) as well as TNFα (concentrations 1 ng/ml; 10 ng/ml and 10 ng/ml and 1 μg/ml actinomycin D) directly in tumour tissue after 6 h, 12 h and 24 h. The apoptotic effect was assessed immunohistochemically. Results: Activating CD95 antibodies combined with actino-mycin D led to a significant increase in apoptosis after 12 h. Using TNFα at a high dosage, a significant increase in the apoptosis rate was observed after 6 h and after 12 h in all dosage groups. Conclusions: PCTS can be used to investigate the effect of different apoptotic signals directly in human tumour tissues. TNFα is able to effectively induce apoptosis in liver metastases of colorectal carcinoma. Thus, the extrinsic pathway of apoptosis may be a promising target in the development of new therapeutic approaches.