RT Journal Article SR Electronic T1 Comparative Study of Biological Activity of Three Commercial Products of Sasa senanensis Rehder Leaf Extract JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 259 OP 264 VO 26 IS 2 A1 HIROSHI SAKAGAMI A1 SHOKO IWAMOTO A1 TOMOHIKO MATSUTA A1 KAZUE SATOH A1 CHIYAKO SHIMADA A1 TAISEI KANAMOTO A1 SHIGEMI TERAKUBO A1 HIDEKI NAKASHIMA A1 YURIKA MORITA A1 ATSUKO OHKUBO A1 TADASHI TSUDA A1 KATSUYOSHI SUNAGA A1 MADOKA KITAJIMA A1 HIROSHI OIZUMI A1 TAKAAKI OIZUMI YR 2012 UL http://iv.iiarjournals.org/content/26/2/259.abstract AB Background: We have previously reported that alkaline extract of Sasa senanensis leaves (SE) has several biological activities characteristic of lignin-carbohydrate complex (LCC). In the present study, we compared the biological activity of three commercially available products of SE (products A, B and C). Materials and Methods: Cell viability of mock-infected, HIV-infected, UV-irradiated cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Radical intensity was determined by electron spin resonance spectroscopy. Cytochrome P-450 (CYP)3A4 activity was measured by β-hydroxylation of testosterone in human recombinant CYP3A4. Results: Product A is a pure SE that contains Fe(II)-chlorophyllin, whereas products B and C contain Cu(II)-chlorophyllin and less LCC. Product C is supplemented with ginseng and pine (Pinus densiflora) leaf extracts. Product A exhibited 5-fold higher anti-HIV, 4-fold higher anti-UV, 5-fold higher hydroxyl radical-scavenging, and 3-fold lower CYP3A4 inhibitory activities as compared to those of product B, and 5-fold higher, 1.5-fold higher, comparable, and 7-fold lower activities, respectively, as compared to those of product C. Conclusion: The present study demonstrates for the first time the superiority of product A over products B and C, suggesting the beneficial role of LCC and Fe(II)-chlorophyllin.