RT Journal Article
SR Electronic
T1 Imbalance of Tumor Suppression Genes Expression Following Rat Tongue Carcinogenesis Induced by 4-Nitroquinoline 1-Oxide
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 937
OP 942
VO 23
IS 6
A1 RIBEIRO, DANIEL ARAKI
A1 FRACALOSSI, ANA CAROLINA CUZZUOL
A1 UATARI, SILVIA AKEMI
A1 OSHIMA, CELINA TIZUKO FUJIYAMA
A1 SALVADORI, DAISY MARIA FAVERO
YR 2009
UL http://iv.iiarjournals.org/content/23/6/937.abstract
AB This study was undertaken to investigate, by immunohistochemistry, the expression of some tumor suppressor genes such as p16, p21 and Retinoblastoma (Rb) during 4-Nitroquinoline 1-oxide induced rat tongue carcinogenesis. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12 or 20 weeks. Ten animals were used as negative control. Neither histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure, nor statistically significant differences (p>0.05) in expression of all the tumor suppressor genes were found when compared to the negative control. However, the levels of Rb were increased (p<0.05) in pre-neoplastic lesions at 12 weeks following carcinogen exposure. In well-differentiated squamous cell carcinoma induced after 20 weeks of treatment with 4NQO, p16 and Rb were expressed in some tumor cells. Taken together, the results support the belief that the expression of Rb is closely event-related to malignant transformation and conversion of the oral mucosa, being a reliable biomarker linked to oral cancer pathogenesis.