RT Journal Article
SR Electronic
T1 Expression of Breast Cancer Resistance Protein (BCRP-1) in Canine Mammary Adenocarcinomas and Adenomas
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 705
OP 709
VO 23
IS 5
A1 NOWAK, MARCIN
A1 MADEJ, JANUSZ A.
A1 DZIEGIEL, PIOTR
YR 2009
UL http://iv.iiarjournals.org/content/23/5/705.abstract
AB Background: Among the recognized factors which induce multi-drug resistance, an increased activity of proteins belonging to the ATP-binding casette family, including breast cancer resistance protein 1 (BCRP-1), is regarded as the most important. Localization and intensity of BCRP-1 expression was evaluated in mammary adenocarcinomas and adenomas in dogs. The obtained results were compared to the grade of malignancy (G) of the tumours. Materials and Methods: Materials for the study were sampled in the course of surgery from 54 dogs, of various breeds, aged 6 to 16 years (36 cases of mammary adenocarcinoma and 18 cases of mammary adenoma). The tumours were histopathologically verified and immunohistochemical reactions were performed to evaluate expression of BCRP-1. The microscopic patterns were photographed and subjected to computer-assisted analysis taking advantage of MultiScanBase Ver. 14.02 software. Results: Expression of BCRP-1 was detected in over 85% of adenocarcinomas and almost 28% of adenomas. Samples of tumours with a higher grade of malignancy demonstrated an increased expression of BCRP-1. The two variables manifested a moderate positive correlation (r=0.35; p<0.05). Conclusion: The results point to a role of BCRP-1 protein in biology of tumour cells in dogs.