PT - JOURNAL ARTICLE AU - AN-CHENG HUANG AU - SHU-CHUN HSU AU - CHAO-LIN KUO AU - CHING-LUNG LIAO AU - KUANG-CHI LAI AU - TSUNG-PING LIN AU - SHIN-HWAR WU AU - HSU-FENG LU AU - NOU-YING TANG AU - JAI-SING YANG AU - JING-GUNG CHUNG TI - Involvement of Matrix Metalloproteinases in the Inhibition of Cell Invasion and Migration Through the Inhibition of NF-κB by the New Synthesized Ethyl 2-[<em>N</em>-<em>p</em>-chlorobenzyl-(2′-methyl)]anilino-4-oxo-4,5-dihydrofuran-3-carboxylate (JOTO1007) in Human Cervical Cancer Ca Ski Cells DP - 2009 Jul 01 TA - In Vivo PG - 613--619 VI - 23 IP - 4 4099 - http://iv.iiarjournals.org/content/23/4/613.short 4100 - http://iv.iiarjournals.org/content/23/4/613.full SO - In Vivo2009 Jul 01; 23 AB - JOTO1007 (ethyl 2-[N-p-chlorobenzyl-(2′-methyl)] anilino-4-oxo-4,5-dihydrofuran -3-carboxylate) has anticancer effects in human cervical cancer Ca Ski cells. However, its mechanism of action on the cell migration and invasion of human cervical cancer Ca Ski cells is not fully understood. In this study, firstly, the effects of JOTO1007 on the migration and invasion of Ca Ski cells were examined by using matrigel counting. The results showed that JOTO1007 suppressed the migration and invasion of the Ca Ski cells. Secondly, the effect of JOTO1007 on the levels of proteins associated with cell metastasis was examined using Western blotting. The results indicated that JOTO1007 inhibited the levels of son of sevenless homolog 1 (SOS-1), growth factor receptor-bound protein 2 (GRB2), Ras homolog gene family, member A (RhoA), Rho-associated, coiled-coil containing protein kinase 1 (ROCK-1), focal adhesion kinase (FAK), phosphorylated-c-jun (p-c-jun), nuclear factor kappa B (NF-κB) p65, cyclooxygenase-2 (COX-2), extracellular signal-regulated kinases 1/2 (ERK1/2), matrix metalloproteinase-2 (MMP-2), MMP-7 and MMP-9 but promoted the levels of protein kinase C (PKC), phosphoinositide 3-kinases (PI3K), MAP kinase kinase kinase 3 (MEKK3), mitogen-activated protein kinase kinase 7 (MKK7), c-jun and inducible nitric oxide synthases (iNOS), while not affecting Ras, phosphorylated-ERK (p-ERK), p38 and c-jun N-terminal kinase 1/2 (JNK1/2), which finally led to the inhibition of migration and invasion of the Ca Ski cells in vitro. Overall, JOTO1007 inhibited NF-κB which then led to the inhibition of the MMP-2, -7 and -9 expression followed by the inhibition of migration and invasion in the Ca Ski cells.