RT Journal Article SR Electronic T1 Fermented Lingonberry Juice Inhibits Oral Tongue Squamous Cell Carcinoma Invasion In Vitro Similarly to Curcumin JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 1089 OP 1095 DO 10.21873/invivo.11350 VO 32 IS 5 A1 HOORNSTRA, DOUWE A1 VESTERLIN, JENNI A1 PÄRNÄNEN, PIRJO A1 AL-SAMADI, AHMED A1 ZLOTOGORSKI-HURVITZ, AYELET A1 VERED, MARILENA A1 SALO, TUULA YR 2018 UL http://iv.iiarjournals.org/content/32/5/1089.abstract AB Background: Oral tongue squamous cell carcinoma (OTSCC) cells are highly proliferative and invasive. Lingonberry contains several polyphenolic compounds similar to curcumin. We hypothesize that fermented lingonberry juice (FLJ) has an anti-invasive and anti-proliferative effect on OTSCC cells similarly to curcumin, which is known to be anti-carcinogenic. Materials and Methods: FLJ, curcumin dissolved in ethanol, or curcumin loaded in Candida extracellular vesicles (EVs) were added to more (HSC-3) and less aggressive (SCC-25) OTSCC cells. Cell proliferation was measured with a 5-bromo-2’-deoxyuridine kit and invasion in the three-dimensional Myogel spheroid assay. Statistical analyses were completed with one-way ANOVA and Bonferroni post-hoc testing. Results: Both FLJ and curcumin significantly reduced the proliferation and invasion of HSC-3 and SCC-25 cells. The effects of curcumin were not improved when cells were treated with curcumin loaded within EVs. Conclusion: Our results suggest that FLJ, like curcumin, has an anti-carcinogenic effect on aggressive OTSCC cells in vitro.