PT  - JOURNAL ARTICLE
AU  - ANTOANETA IVANOVA
AU  - JULIANNA SERLY
AU  - DRAGOMIR DINCHEV
AU  - IMRE OCSOVSZKI
AU  - IVANKA KOSTOVA
AU  - JOSEPH MOLNAR
TI  - Screening of Some Saponins and Phenolic Components of <em>Tribulus terrestris</em> and <em>Smilax excelsa</em> as MDR Modulators
DP  - 2009 Jul 01
TA  - In Vivo
PG  - 545--550
VI  - 23
IP  - 4
4099  - http://iv.iiarjournals.org/content/23/4/545.short
4100  - http://iv.iiarjournals.org/content/23/4/545.full
SO  - In Vivo2009 Jul 01; 23
AB  - Background: Cytotoxic activity of saponins and phenolic compounds have been described in the literature, but no reports were found on their multidrug resistance (MDR)-modulating effects on human mdr1 gene-transfected mouse lymphoma cell line. Materials and Methods: Methylprototribestin, structurally related compounds and a mixture of 3 acetylated isomers of methylprotodioscin were investigated for antiproliferative effect and modulation of drug accumulation. Results: The growth inhibitory dose (ID50) of the compounds ranged from 12.64 to 20.62 μg/ml. Methylprototribestin was the most effective resistance modifier. However, methylprotodioscin, pseudoprotodioscin, prosapogenin A of dioscin, tribestin and 5-O-caffeoylshikimic acid showed moderate MDR reversal activity. In a checkerboard method, methyloprototribestin and the mixture of the 3 acetylated isomers enhanced the antiproliferative effects on MDR cells in combination with doxorubicin. Conclusion: Based on these results, methylprototribestin and the mixture of the 3 acetylated isomers can be recommended for further in vivo experiments in combination with anthracyclines in human MDR-cancer xenograft transplanted mice.