RT Journal Article
SR Electronic
T1 Antiviral Activities of Extracts of <em>Euphorbia hirta</em> L. against HIV-1, HIV-2 and SIV<sub>mac251</sub>
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 429
OP 432
VO 23
IS 3
A1 GYURIS, ÁGNES
A1 SZLÁVIK, LÁSZLÓ
A1 MINÁROVITS, JÁNOS
A1 VASAS, ANDREA
A1 MOLNÁR, JOSEPH
A1 HOHMANN, JUDIT
YR 2009
UL http://iv.iiarjournals.org/content/23/3/429.abstract
AB The antiretroviral activities of extracts of Euphorbia hirta were investigated in vitro on the MT4 human T lymphocyte cell line. The cytotoxicities of the extracts were tested by means of the MTT cell proliferation assay, and then the direct effects of the aqueous extract on HIV-1, HIV-2 and SIVmac251 reverse transcriptase (RT) activity were determined. A dose-dependent inhibition of RT activity was observed for all three viruses. The HIV-1 inhibitory potency of E. hirta was studied further, and the activities of the aqueous and 50% methanolic extracts were compared. The 50% methanolic extract was found to exert a higher antiretroviral effect than that of the aqueous extract. The 50% MeOH extract was subjected to liquid-liquid partition with dichloromethane, ethyl acetate and water. Only the remaining aqueous phase exhibited significant antiviral activity; all the lipophilic extracts appeared to be inactive. After removal of the tannins from the aqueous extract, the viral replication inhibitory effect was markedly decreased, and it was therefore concluded that tannins are most probably responsible for the high antiretroviral activity.