@article {RUBIO599, author = {CARLOS A. RUBIO}, title = {An Easy Method to Identify Parietal Cells in Gastric Biopsies}, volume = {24}, number = {4}, pages = {599--602}, year = {2010}, publisher = {International Institute of Anticancer Research}, abstract = {Background: Sections from gastric biopsies are usually stained with hematoxylin and eosin (H\&E), a stain that is not optimal for the recognition of many parietal cells. This paper describes a more suitable routine stain to identify parietal cells. Patients and Methods: Nineteen sets of gastric biopsies were consecutively stained with H\&E and with modified Giemsa. Giemsa-stained fundic biopsies showed a parietal cell band intercalated between the fovelar epithelium and the chief glands. The continuity of this band was studied at {\texttimes}4 magnification and its thickness in one well-oriented field at {\texttimes}10 magnification. Results: A distinct, continuous parietal cell band was recorded in fundic biopsies exhibiting normal mucosa, acute gastritis or chronic gastritis without glandular atrophy (Group A). A discontinuous or lack of parietal cell band was found in fundic biopsies exhibiting chronic gastritis with glandular atrophy or with intestinal metaplasia (Group B). The ratio of parietal cell band/total mucosal thickness ranged between 0.30 and 0.40 in Group A and between 0 and 0.25 in Group B. Conclusion: A parietal cell band was readily demonstrated in sections from gastric biopsies stained with Giemsa, but not in those stained with H\&E. Discontinuity with reduced or absent band was recorded in gastric diseases characterised by a decrease of the parietal cell population.}, issn = {0258-851X}, URL = {https://iv.iiarjournals.org/content/24/4/599}, eprint = {https://iv.iiarjournals.org/content/24/4/599.full.pdf}, journal = {In Vivo} }