PT - JOURNAL ARTICLE AU - BÁRBARA DO NASCIMENTO BORGES AU - ROMMEL MARIO RODRIGUEZ BURBANO AU - MARIA LÚCIA HARADA TI - Absence of <em>CIP1/KIP1</em> Hypermethylation in Gastric Cancer Patients from Northern Brazil DP - 2010 Jul 01 TA - In Vivo PG - 579--582 VI - 24 IP - 4 4099 - http://iv.iiarjournals.org/content/24/4/579.short 4100 - http://iv.iiarjournals.org/content/24/4/579.full SO - In Vivo2010 Jul 01; 24 AB - The aim of this study was to investigate the protein expression and methylation pattern of P21CIP1 and P27KIP1 genes. Patients and Methods: Twenty samples of gastric tumor and non-tumoral tissues of patients from Pará state, Brazil were collected. Methylation patterns were assessed by bisulfite sequencing, and protein expression evaluated with immunohistochemical analysis. Results: None of the analyzed samples showed methylation in both genes. Immunohistochemistry analysis demonstrated mislocalization or absence of expression of P21CIP1 and P27KIP1 in 7/20 and 6/20 of the studied samples, respectively. No correlations regarding protein expression and clinicopathological characteristics were observed; down-regulation of expression of P21CIP1 with low (I-II) tumor stage (p=0.0777), and older age (&gt;50 years old) with negative or mislocalization of P27KIP1 (p=0.0922) were of borderline the statistical significance. Conclusion: Our results suggest that hypermethylation does not contribute to P21CIP1 and P27KIP1 silencing in gastric cancer, and that the role of these genes in the gastric tumorigenesis pathways should be studied further in the Pará state population.