@article {DO NASCIMENTO BORGES579, author = {B{\'A}RBARA DO NASCIMENTO BORGES and ROMMEL MARIO RODRIGUEZ BURBANO and MARIA L{\'U}CIA HARADA}, title = {Absence of CIP1/KIP1 Hypermethylation in Gastric Cancer Patients from Northern Brazil}, volume = {24}, number = {4}, pages = {579--582}, year = {2010}, publisher = {International Institute of Anticancer Research}, abstract = {The aim of this study was to investigate the protein expression and methylation pattern of P21CIP1 and P27KIP1 genes. Patients and Methods: Twenty samples of gastric tumor and non-tumoral tissues of patients from Par{\'a} state, Brazil were collected. Methylation patterns were assessed by bisulfite sequencing, and protein expression evaluated with immunohistochemical analysis. Results: None of the analyzed samples showed methylation in both genes. Immunohistochemistry analysis demonstrated mislocalization or absence of expression of P21CIP1 and P27KIP1 in 7/20 and 6/20 of the studied samples, respectively. No correlations regarding protein expression and clinicopathological characteristics were observed; down-regulation of expression of P21CIP1 with low (I-II) tumor stage (p=0.0777), and older age (\>50 years old) with negative or mislocalization of P27KIP1 (p=0.0922) were of borderline the statistical significance. Conclusion: Our results suggest that hypermethylation does not contribute to P21CIP1 and P27KIP1 silencing in gastric cancer, and that the role of these genes in the gastric tumorigenesis pathways should be studied further in the Par{\'a} state population.}, issn = {0258-851X}, URL = {https://iv.iiarjournals.org/content/24/4/579}, eprint = {https://iv.iiarjournals.org/content/24/4/579.full.pdf}, journal = {In Vivo} }