RT Journal Article
SR Electronic
T1 Type of Cell Death Induced by Seven Metals in Cultured Mouse Osteoblastic Cells
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 507
OP 512
VO 24
IS 4
A1 RENÉ GARCÍA CONTRERAS
A1 JOSÉ ROGELIO SCOUGALL VILCHIS
A1 HIROSHI SAKAGAMI
A1 YUKO NAKAMURA
A1 YUKIO NAKAMURA
A1 YASUSHI HIBINO
A1 HIROSHI NAKAJIMA
A1 JUN SHIMADA
YR 2010
UL http://iv.iiarjournals.org/content/24/4/507.abstract
AB The use of dental metal alloys in the daily clinic makes it necessary to evaluate the cytotoxicity of eluted metal components against oral cells. However, the cytotoxic mechanism and the type of cell death induced by dental metals in osteoblasts have not been well characterized. This study investigated the cytotoxicity of seven metals against the mouse osteoblastic cell line MC3T3-E1. α-MEM was used as a culture medium, since this medium provided much superior proliferation of MC3T3-E1 cells over DMEM. Ag (NH3)2F was the most cytotoxic, followed by CuCl>CuCl2 >CoCl2, NiCl2>FeCl3 and FeCl2 (least toxic). None of the metals showed any apparent growth stimulating effect (so-called ‘hormesis’) at lower concentrations. A time course study demonstrated that two hours of contact between oral cells and Ag (NH3)2F, CuCl, CoCl2 or NiCl2 induced irreversible cell death. Contact with these metals induced a smear pattern of DNA fragmentation without activation of caspase-3. Preincubation of MC3T3-E1 cells with either a caspase inhibitor (Z-VAD-FMK) or autophagy inhibitors (3-methyladenine, bafilomycin) failed to rescue them from metal cytotoxicity. These data suggest the induction of necrotic cell death rather than apoptosis and autophagy by metals in this osteoblastic cell line.