RT Journal Article SR Electronic T1 Vanillin Enhances TRAIL-Induced Apoptosis in Cancer Cells through Inhibition of NF-κB Activation JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 501 OP 506 VO 24 IS 4 A1 KRIENGSAK LIRDPRAPAMONGKOL A1 HIROAKI SAKURAI A1 SHUNSUKE SUZUKI A1 KEIICHI KOIZUMI A1 ORAWIN PRANGSAENGTONG A1 AMORNRAT VIRIYAROJ A1 SOMSAK RUCHIRAWAT A1 JISNUSON SVASTI A1 IKUO SAIKI YR 2010 UL http://iv.iiarjournals.org/content/24/4/501.abstract AB Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent which selectively kills cancer cells with little effect on normal cells. However, TRAIL resistance is widely found in cancer cells. We have previously reported antimetatstatic and antiangiogenic effects of vanillin, a flavoring agent from vanilla. Here we have evaluated the sensitizing effect of vanillin on a TRAIL-resistant human cervical cancer cell line, HeLa. Materials and Methods: Cell viability after treatments was determined by the WST-1 cell counting kit. Apoptosis was demonstrated by detection of caspase-3 activation and cleavage of poly (ADP-ribose) polymerase using immunoblot analysis. Effect of treatments on TRAIL signaling pathway and nuclear factor κB (FN-κB) activation was studied using immunoblot analysis and luciferase reporter assay. Results: Pretreatment of HeLa cells with vanillin enhanced TRAIL-induced cell death through the apoptosis pathway. Vanillin pretreatment inhibited TRAIL-induced phosphorylation of p65 and transcriptional activity of NF-κB. Conclusion: Vanillin sensitizes HeLa cells to TRAIL-induced apoptosis by inhibiting NF-κB activation.