RT Journal Article
SR Electronic
T1 Vanillin Enhances TRAIL-Induced Apoptosis in Cancer Cells through Inhibition of NF-κB Activation
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 501
OP 506
VO 24
IS 4
A1 LIRDPRAPAMONGKOL, KRIENGSAK
A1 SAKURAI, HIROAKI
A1 SUZUKI, SHUNSUKE
A1 KOIZUMI, KEIICHI
A1 PRANGSAENGTONG, ORAWIN
A1 VIRIYAROJ, AMORNRAT
A1 RUCHIRAWAT, SOMSAK
A1 SVASTI, JISNUSON
A1 SAIKI, IKUO
YR 2010
UL http://iv.iiarjournals.org/content/24/4/501.abstract
AB Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent which selectively kills cancer cells with little effect on normal cells. However, TRAIL resistance is widely found in cancer cells. We have previously reported antimetatstatic and antiangiogenic effects of vanillin, a flavoring agent from vanilla. Here we have evaluated the sensitizing effect of vanillin on a TRAIL-resistant human cervical cancer cell line, HeLa. Materials and Methods: Cell viability after treatments was determined by the WST-1 cell counting kit. Apoptosis was demonstrated by detection of caspase-3 activation and cleavage of poly (ADP-ribose) polymerase using immunoblot analysis. Effect of treatments on TRAIL signaling pathway and nuclear factor κB (FN-κB) activation was studied using immunoblot analysis and luciferase reporter assay. Results: Pretreatment of HeLa cells with vanillin enhanced TRAIL-induced cell death through the apoptosis pathway. Vanillin pretreatment inhibited TRAIL-induced phosphorylation of p65 and transcriptional activity of NF-κB. Conclusion: Vanillin sensitizes HeLa cells to TRAIL-induced apoptosis by inhibiting NF-κB activation.