RT Journal Article
SR Electronic
T1 Small Molecule GS-Nitroxide Ameliorates Ionizing Irradiation-Induced Delay in Bone Wound Healing in a Novel Murine Model
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 377
OP 385
VO 24
IS 4
A1 ABHAY GOKHALE
A1 JEAN-CLAUDE RWIGEMA
A1 MICHAEL W. EPPERLY
A1 JULIE GLOWACKI
A1 HONG WANG
A1 PETER WIPF
A1 JULIE P. GOFF
A1 TRACY DIXON
A1 KEN PATRENE
A1 JOEL S. GREENBERGER
YR 2010
UL http://iv.iiarjournals.org/content/24/4/377.abstract
AB We studied radioprotection and mitigation by mitochondrial-targeted Tempol (GS-nitroxide, JP4-039), in a mouse injury/irradiation model of combined injury (fracture/irradiation). Right hind legs of control C57BL/6NHsd female mice, mice pretreated with MnSOD-PL, JP4-039, or with amifostine were irradiated with single and fractionated doses of 0 to 20 Gy. Twenty-four hours later, unicortical holes were drilled into the tibiae of both hind legs; at intervals, tibias were excised, radiographed, and processed for histology. Bone wounds irradiated to 20 or 30 Gy showed delayed healing at 21 to 28 days. Treatment with JP4-039 MnSOD-PL or amifostine, before or after single fraction 20 Gy or during fractionated irradiation followed by drilling accelerated wound healing at days 21 and 28. Orthotopic 3LL tumors were not protected by JP4-039 or amifostine. In nonirradiated mice, pretreatment with JP4-039 accelerated bone wound healing. This test system should be useful for the development of new small molecule radioprotectors.