RT Journal Article SR Electronic T1 Comparison of Lipid Profiles in Relation to APOB EcoRI Polymorphism in Obese Children with Hyperlipidemia JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 65 OP 69 VO 24 IS 1 A1 OZLEM TİMİRCİ A1 FEYZA DARENDELİLER A1 FİRDEVS BAS A1 ERGEN H. ARZU A1 ZEYBEK UMİT A1 TURGAY ISBİR YR 2010 UL http://iv.iiarjournals.org/content/24/1/65.abstract AB Background: We aimed to evaluate apolipoprotein B-100 (APOB) EcoRI polymorphism and plasma lipid parameters together in children and adolescents. This is the first such study in Turkey to determine possible relationships of these parameters. Materials and Methods: Three separate groups were studied: a group of obese children with hyperlipidemia, a group of obese children without hyperlipidemia, and a group of healthy children neither with hyperlipidemia nor obesity. Polymerase chain reaction (PCR), denaturating gradient gel electrophoresis (DGGE) and automatic sequence analysis techniques were used. Sequencing results were examined by Proseq and BioEdit computer programmes. Results: Mutant A allele was not observed in the healthy control group, whereas it was more frequent in the hyperlipidemic obese children; the GA genotype was correlated with total and low density lipoprotein-cholesterol levels. Conclusion: In this study, we suggest that obese child patients having the A allele could have a higher risk for developing hyperlipidemia. Copyright © 2010 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved