RT Journal Article SR Electronic T1 Effect of Contact with Titanium Alloys on the Proliferation of Mouse Osteoblastic Cells in Culture JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 29 OP 37 VO 24 IS 1 A1 HIROYUKI ONUKI A1 HIROSHI SAKAGAMI A1 MASAHIKO KOBAYASHI A1 YASUSHI HIBINO A1 YOSHIKO YOKOTE A1 HIROSHI NAKAJIMA A1 JUN SHIMADA YR 2010 UL http://iv.iiarjournals.org/content/24/1/29.abstract AB This study was aimed at studying the effect of contact with titanium alloy plates of different surface textures on the proliferative capability of mouse osteoblastic MC3T3-E1 cells. First, the proliferation characteristics of MC3T3-E1 cells were investigated. MC3T3-E1 cells showed a high capacity for proliferation and survived for a long period even under nutritionally starved conditions. During logarithmic cell growth, the consumption of Ser, Gln, Val, Ile and Leu increased time-dependently. Contact with an hydoxyapatite (HA)-coated titanium alloy plate resulted in the increase in the recovery of cells from the plate by trypsin, and an increase in the consumption of these amino acids, suggesting enhanced cell proliferation. On the contrary, contact with the sandblasted and anodized titanium alloy plates resulted in the reduction of the recovery of the cells from the plate, but a slight increase in the amino acid consumption, suggesting the tight adhesion of the cells to the plates. This study demonstrates that the present method, based on the amino acid consumption of the cells, is useful for monitoring the cell proliferative capability, without detachment of the cells from the plate. This method may be applicable to the study of the interaction between cells and metal plates. Copyright © 2010 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved