TY - JOUR T1 - Inhibitory Effects of Decoy-ODN Targeting Activated STAT3 on Human Glioma Growth <em>In Vivo</em> JF - In Vivo JO - In Vivo SP - 237 LP - 243 VL - 23 IS - 2 AU - JIE SHEN AU - RONGHUI LI AU - GANG LI Y1 - 2009/03/01 UR - http://iv.iiarjournals.org/content/23/2/237.abstract N2 - Background: It has been previously found that a constitutively activated signal transducer and activator of transcription 3 (STAT3) blocked by decoy-ODN led to glioma growth inhibition in vitro. The objectives of this study were to identify whether STAT3 decoy-ODN had the same effect or not in vivo. Materials and Methods: Western blot was used to detect p-STAT3 in glioma and normal brain tissue. U251 cells were subcutaneously injected into nude mice and decoy-ODN was intratumorally administrated. TUNEL was used to exam the apoptosis cells in xenografts. Genes regulated by STAT3 were evaluated by RT-PCR and immunohistochemistry. Results: Activated STAT3 was highly present in glioma but not normal brain tissues. STAT3 decoy-ODN could significantly suppress the growth of glioma by inhibiting proliferation and promoting apoptosis in xenografts. The target genes controlled by STAT3 were down-regulated at both transcription and translation levels. Conclusion: The present study suggested that decoy-ODN provide an effective therapeutic approach to treat glioma in vivo. ER -