PT  - JOURNAL ARTICLE
AU  - YOONYOUNG CHUNG
AU  - HANBIT CHO
AU  - GYEONG HYEON JO
AU  - YONGHYUN JUN
TI  - Hypoxia of Rats Subjected to Carotid Artery Ligation Results in Impaired Neurogenesis and Reduced Number of Cortical Neurons
AID  - 10.21873/invivo.11492
DP  - 2019 Mar 01
TA  - In Vivo
PG  - 441--445
VI  - 33
IP  - 2
4099  - http://iv.iiarjournals.org/content/33/2/441.short
4100  - http://iv.iiarjournals.org/content/33/2/441.full
SO  - In Vivo2019 Mar 01; 33
AB  - Background/Aim: Cerebral ischemia is a major cause of abnormal brain development. In a cerebral ischemia model, periventricular leukomalacia (PVL), white matter lesion and a decrease in the number of subcortical neurons were observed. The aim of this study was to investigate the effect of hypoxia on neurogenesis and cell survival. Materials and Methods: In seven-day postnatal rats, the right carotid artery was ligated. The rats were incubated either in a regular normoxic chamber (control group) or in a hypoxic chamber (PVL group, 8% 02 and 92% N2 at 37°C) for 2 h. Nestin- and NeuN-positive neurons were detected by immunohistochemistry. Results: The densities of nestin-immunoreactivity (IR) cells in the cerebral parietal cortex and subventricular zone were increased with hypoxia. NeuN-IR cells in the cerebral cortex were significantly decreased in the PVL group. Conclusion: Perinatal white matter injury induced neurogenesis, while the survival of neurons was decreased in the cerebral cortex.