RT Journal Article
SR Electronic
T1 Fatty Acid Composition of Plasma and Kidney in Rats with Anti-Thy1.1 Nephritis
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 77
OP 79
VO 21
IS 1
A1 RYUICHIRO KUMASAKA
A1 NORIO NAKAMURA
A1 HIDEAKI YAMABE
A1 HIROSHI OSAWA
A1 KEN-ICHI SHIRATO
A1 MICHIKO SHIMADA
A1 REIICHI MURAKAMI
A1 TAKESHI FUJITA
A1 KEN OKUMURA
A1 KEI HAMAZAKI
A1 TOMOHITO HAMAZAKI
YR 2007
UL http://iv.iiarjournals.org/content/21/1/77.abstract
AB Background: n-3 Polyunsaturated fatty acids (PUFA) are reported to ameliorate atherosclerotic and inflammatory diseases because they compete with arachidonic acid and reduce its inflammatory metabolites. In the present study, the fatty acid composition of plasma and kidney in rats with anti-Thy1.1 nephritis was investigated. Materials and Methods: A group of male Wister rats weighing about 200 g was injected with anti-Thy1.1 antibody (1.25 mL/kg) through their tail veins (nephritis group). Rats in the control group were injected with saline. Five days after the injection, urinary protein levels were determined. All rats were then sacrificed and fatty acid composition of plasma and kidney were analyzed. Results: Eicosapentaenoic acids (EPA) levels in the kidney phospholipid (PL) fraction in the nephritis group were significantly lower than those in the control group (0.67±0.06 mol% vs. 0.96±0.06 mol%, p<0.05). EPA levels in the plasma PL fraction in the nephritis group were also significantly lower than those in the control group (0.38±0.05 mol% vs. 0.59±0.03 mol%, p<0.05). Urinary protein levels 5 days after the injection were inversely correlated with EPA levels in the kidney PL fraction (r2=0.65, p=0.01). These results suggested that decreased EPA levels in the kidney PL fraction might play an important role in anti-Thy1.1 nephritis. Copyright © 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved