PT  - JOURNAL ARTICLE
AU  - ALEXANDER SAUTER
AU  - JENS STERN-STRAETER
AU  - SELINA SODHA
AU  - KARL HÖRMANN
AU  - RAMIN NAIM
TI  - Regulation of Matrix Metalloproteinases (MMP)-2/-9 Expression in Eosinophilic Chronic Rhinosinusitis - Cell Culture by Interleukin-5 and -13?
DP  - 2008 Jul 01
TA  - In Vivo
PG  - 415--421
VI  - 22
IP  - 4
4099  - http://iv.iiarjournals.org/content/22/4/415.short
4100  - http://iv.iiarjournals.org/content/22/4/415.full
SO  - In Vivo2008 Jul 01; 22
AB  - Background: Eosinophils are a prominent immunological feature of chronic rhinosinusitis (CRS). Cytokines in the respiratory mucosa may be the key to upper airway pathophysiology. Matrix metalloproteinases (MMP) represent an entire group of Zn2+ dependent endopeptidases with the potential to alter the extracellular matrix (ECM). In this study epithelial cultures of CRS were treated with interleukin (IL)-5 or IL-13 and subsequent levels of metalloproteinases were determined. Materials and Methods: The cells for CRS culture were obtained from patients undergoing functional endoscopic sinus surgery. After 8-72 hours incubation with 0.2-0.4 ng/ml IL-5 or 3-6 ng/ml IL-13, the expression of the MMP-2 and -9 in the CRS cultures was analysed. Results: After 72 hours incubation with IL-5, the relative levels of MMP-2 showed no significant alteration in protein expression in comparison with the control groups. Incubation with IL-13 revealed a statistically insignificant decrease of the relative MMP-9 expression in ECRS compared to the control group (p>0.1). Conclusion: Alterations of MMP-2 and -9 expression may play a role in ECRS, but the association with IL-5 and IL-13 remains unclear.