RT Journal Article
SR Electronic
T1 COX-2 as Possible Target for the Inhibition of PGE<sub>2</sub> Production by <em>Rikko-san</em> in Activated Macrophage
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 333
OP 336
VO 22
IS 3
A1 NORIO HORIE
A1 KEN HASHIMOTO
A1 TAKAO KATO
A1 TETSUO SHIMOYAMA
A1 TADAYOSHI KANEKO
A1 KAORU KUSAMA
A1 HIROSHI SAKAGAMI
YR 2008
UL http://iv.iiarjournals.org/content/22/3/333.abstract
AB We have previously reported that Rikko-san (RKS) inhibited the lipopolysaccharide (LPS)-stimulated prostaglandin (PG) E2 in mouse macrophage-like RAW264.7 cells without affecting the expression of cyclooxygenase (COX)-2. Here RKS inhibition of the enzyme activity of both COX-1 and COX-2 proteins was investigated. Western blot analysis showed that RKS did not significantly change the S-nitrosylated COX-2 protein level. On the other hand, RKS inhibited the PG production catalyzed by purified COX-2, more effectively than that catalyzed by purified COX-1. These results suggest that RKS inhibits the PGE2 production by selectively inhibiting the COX-2 activity in activated macrophages.