@article {HORIE333, author = {NORIO HORIE and KEN HASHIMOTO and TAKAO KATO and TETSUO SHIMOYAMA and TADAYOSHI KANEKO and KAORU KUSAMA and HIROSHI SAKAGAMI}, title = {COX-2 as Possible Target for the Inhibition of PGE2 Production by Rikko-san in Activated Macrophage}, volume = {22}, number = {3}, pages = {333--336}, year = {2008}, publisher = {International Institute of Anticancer Research}, abstract = {We have previously reported that Rikko-san (RKS) inhibited the lipopolysaccharide (LPS)-stimulated prostaglandin (PG) E2 in mouse macrophage-like RAW264.7 cells without affecting the expression of cyclooxygenase (COX)-2. Here RKS inhibition of the enzyme activity of both COX-1 and COX-2 proteins was investigated. Western blot analysis showed that RKS did not significantly change the S-nitrosylated COX-2 protein level. On the other hand, RKS inhibited the PG production catalyzed by purified COX-2, more effectively than that catalyzed by purified COX-1. These results suggest that RKS inhibits the PGE2 production by selectively inhibiting the COX-2 activity in activated macrophages.}, issn = {0258-851X}, URL = {https://iv.iiarjournals.org/content/22/3/333}, eprint = {https://iv.iiarjournals.org/content/22/3/333.full.pdf}, journal = {In Vivo} }