TY - JOUR T1 - Expression in Childhood Primary Brain Tumors of NY-ESO-1, a Cancer/Testis Antigen: An Immunohistochemical Study JF - In Vivo JO - In Vivo SP - 83 LP - 87 VL - 22 IS - 1 AU - BELA BODEY AU - VIVIAN BODEY AU - STUART E. SIEGEL Y1 - 2008/01/01 UR - http://iv.iiarjournals.org/content/22/1/83.abstract N2 - Background: NY-ESO-1 is a human gene that codes for antigens that are expressed in malignancies of various histological types, but not in normal tissues, except the testes. The expression of NY-ESO-1 in intracranial brain tumors including astrocytomas (ASTRs) and medulloblastomas (MEDs)/primitive neuroectodermal tumors (PNETs) was examined since the expression of NY-ESO-1 has only previously been explored in depth in neuroblastomas. Materials and Methods: During our immunohistochemical study, a sensitive, four-step, alkaline phosphatase-conjugated antigen detection technique was employed. The expression of NY-ESO-1 was thereby examined in 6 cases of MED/PNET and 14 cases of ASTR. Results: All 6 MED/PNET cases demonstrated high levels of immunoreactivity (overexpression) with the highest immunostaining intensity grades A and B. In the astrocytic tumors of various subtypes examined, the level of NY-ESO-1 expression was not as strong as that in MEDs/PNETs. However, there was a significant increase in expression level when comparing low-grade pilocytic ASTRs to high-grade anaplastic ASTRs and glioblastomas. Conclusion: As evidenced by our results, NY-ESO-1 overexpression increases as the malignancy grade of the astrocytic tumors increases. These data suggest that antigen-directed immunotherapy of primary brain tumors could target cancer/testis antigens (CTAs), especially those expressed at higher frequency such as NY-ESO-1. Copyright © 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -