RT Journal Article
SR Electronic
T1 Antitumor Activity of Betulinic Acid and Betulin in Canine Cancer Cell Lines
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 1081
OP 1088
DO 10.21873/invivo.11349
VO 32
IS 5
A1 JING ZHAO
A1 RONGFANG LI
A1 ALEKSANDRA PAWLAK
A1 MARTA HENKLEWSKA
A1 ANGELIKA SYSAK
A1 LIXIN WEN
A1 JIN-E YI
A1 BOŻENA OBMIŃSKA-MRUKOWICZ
YR 2018
UL http://iv.iiarjournals.org/content/32/5/1081.abstract
AB Background/Aim: Betulinic acid (BA) and betulin (BT) exhibit a variety of pharmacological properties including anti-cancer, anti-inflammatory and anti-oxidant ones. Canine lymphoma and osteosarcoma have a high mortality rate and need more effective therapeutic approaches. In this study, the anti-proliferative and pro-apoptotic effects of BA and BT were investigated in canine T-cell lymphoma (CL-1), canine B-cell lymphoma (CLBL-1) and canine osteosarcoma (D-17) cell lines. Materials and Methods: The cultured cells were treated with several concentrations of BA or BT for 24, 48 and 72 h, and cell proliferation was assessed by the MTT assay. Cell apoptotic rate and cell cycle were analyzed using flow cytometry. Results: Anti-proliferative effect of BT and BA was concentration- and time-dependent. Moreover, BA and BT arrested cell cycle in S phase in CL-1 and D-17 cells, and in G0/G1 phase in CLBL-1 cells. Conclusion: Both compounds showed an antitumor activity, and the effects of BA were stronger than that of BT.